@article{90766,
  keywords = {Animals, Humans, Light, computational biology, signal transduction, Models, Biological, Protein Binding, Feedback, Physiological, Cell Membrane, Phosphatidylinositol 3-Kinases},
  author = {Jared Toettcher and Delquin Gong and Wendell Lim and Orion Weiner},
  title = {Light-based feedback for controlling intracellular signaling dynamics.},
  abstract = { The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an {\textquoteright}optogenetic{\textquoteright} method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity. },
  year = {2011},
  journal = {Nat Methods},
  volume = {8},
  pages = {837-9},
  month = {09/2011},
  issn = {1548-7105},
  doi = {10.1038/nmeth.1700},
  language = {eng},
}