Light-based feedback for controlling intracellular signaling dynamics.
Publication Year
2011
Type
Journal Article
Abstract
The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.
Keywords
Journal
Nat Methods
Volume
8
Issue
10
Pages
837-9
Date Published
09/2011
ISSN Number
1548-7105
Alternate Journal
Nat. Methods
PMID
21909100